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alphalisa signal  (Revvity)


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    Revvity alphalisa signal
    Alphalisa Signal, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 13581 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+signal/pmc13114140-83-1-9?v=Revvity
    Average 96 stars, based on 13581 article reviews
    alphalisa signal - by Bioz Stars, 2026-07
    96/100 stars

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    (A-B) Normalized p-eIF4E <t>AlphaLisa</t> count from (A) cortical lysate and (B) synaptoneurosomes from wild-type, MNK1 KO , and MNK2 KO mice. MNK1/2 DKO are included as validation. (C-D) Quantification of eIF4E from wild-type, MNK1 KO , MNK2 KO , and MNK1/2 DKO mice in (C) cortical lysate and (D) synaptoneurosomes. Images are in . (E) Representative image and quantification of p-4EBP1 compared to total 4EBP1 in synaptoneurosomes from wild-type, MNK1 KO , and MNK2 KO mice. (F-G) Representative image and quantification p-ERK1/2 compared to total ERK1/2 in wild-type, MNK1 KO , MNK2 KO , and MNK1/2 DKO mice in (F) cortical lysate and (G) synaptoneurosomes. (H-I) Representative image and quantification of MNK1 in wild-type and MNK2 KO mice in (H) cortical lysate and (I) synaptoneurosomes. MNK1 KO and MNK1/2 DKO mice are included as validation. Significance was determined by Kruskal-Wallis test followed by Dunn’s multiple comparison test for A-B, one-way ANOVA for C-G, and Mann-Whitney test for H-I.
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    (A-B) Normalized p-eIF4E AlphaLisa count from (A) cortical lysate and (B) synaptoneurosomes from wild-type, MNK1 KO , and MNK2 KO mice. MNK1/2 DKO are included as validation. (C-D) Quantification of eIF4E from wild-type, MNK1 KO , MNK2 KO , and MNK1/2 DKO mice in (C) cortical lysate and (D) synaptoneurosomes. Images are in . (E) Representative image and quantification of p-4EBP1 compared to total 4EBP1 in synaptoneurosomes from wild-type, MNK1 KO , and MNK2 KO mice. (F-G) Representative image and quantification p-ERK1/2 compared to total ERK1/2 in wild-type, MNK1 KO , MNK2 KO , and MNK1/2 DKO mice in (F) cortical lysate and (G) synaptoneurosomes. (H-I) Representative image and quantification of MNK1 in wild-type and MNK2 KO mice in (H) cortical lysate and (I) synaptoneurosomes. MNK1 KO and MNK1/2 DKO mice are included as validation. Significance was determined by Kruskal-Wallis test followed by Dunn’s multiple comparison test for A-B, one-way ANOVA for C-G, and Mann-Whitney test for H-I.

    Journal: bioRxiv

    Article Title: Distinct roles for MNK1 and MNK2 in social and cognitive behavior through kinase-specific regulation of the synaptic proteome and phosphoproteome

    doi: 10.1101/2024.12.18.628637

    Figure Lengend Snippet: (A-B) Normalized p-eIF4E AlphaLisa count from (A) cortical lysate and (B) synaptoneurosomes from wild-type, MNK1 KO , and MNK2 KO mice. MNK1/2 DKO are included as validation. (C-D) Quantification of eIF4E from wild-type, MNK1 KO , MNK2 KO , and MNK1/2 DKO mice in (C) cortical lysate and (D) synaptoneurosomes. Images are in . (E) Representative image and quantification of p-4EBP1 compared to total 4EBP1 in synaptoneurosomes from wild-type, MNK1 KO , and MNK2 KO mice. (F-G) Representative image and quantification p-ERK1/2 compared to total ERK1/2 in wild-type, MNK1 KO , MNK2 KO , and MNK1/2 DKO mice in (F) cortical lysate and (G) synaptoneurosomes. (H-I) Representative image and quantification of MNK1 in wild-type and MNK2 KO mice in (H) cortical lysate and (I) synaptoneurosomes. MNK1 KO and MNK1/2 DKO mice are included as validation. Significance was determined by Kruskal-Wallis test followed by Dunn’s multiple comparison test for A-B, one-way ANOVA for C-G, and Mann-Whitney test for H-I.

    Article Snippet: AlphaLISA signals were measured using a Tecan SPARK plate reader in the recommended settings.

    Techniques: Biomarker Discovery, Comparison, MANN-WHITNEY

    Principle of AlphaLISA for detecting kidney injury molecule-1.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Development and application of an amplified luminescent proximity homogeneous assay-linked immunosorbent assay for the accurate quantification of kidney injury molecule-1

    doi: 10.3389/fmolb.2023.1280681

    Figure Lengend Snippet: Principle of AlphaLISA for detecting kidney injury molecule-1.

    Article Snippet: The samples were incubated for 20 min at 37°C and shielded from light, and the AlphaLISA signal was detected using Tecan M200.

    Techniques:

    Correlation analysis between the Kim-1-AlphaLISA and Kim-1-TRFIA.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Development and application of an amplified luminescent proximity homogeneous assay-linked immunosorbent assay for the accurate quantification of kidney injury molecule-1

    doi: 10.3389/fmolb.2023.1280681

    Figure Lengend Snippet: Correlation analysis between the Kim-1-AlphaLISA and Kim-1-TRFIA.

    Article Snippet: The samples were incubated for 20 min at 37°C and shielded from light, and the AlphaLISA signal was detected using Tecan M200.

    Techniques: